Journal: Human Reproduction (Oxford, England)

Article Title: The role of small extracellular vesicle-miRNAs in endometriosis

doi: 10.1093/humrep/dead216

Figure Lengend Snippet: Studies investigating the differential expression of sEV-miRNA.

Article Snippet: 13 , Uterine aspirate fluid , Human samples: Endometriosis (stage III–IV, endometrioma, diagnosed via laparoscopy), n=22 Control (including simple ovarian cyst, uterine leiomyoma, tubal ligation), n=25 *Mentioned about that findings were regardless of secretory or proliferative phases in the results section but did not declare number of samples that were of proliferative or secretory. , Size exclusion chromatography exosome isolation kit (Echobiotech, Beijing, China) , NTA : Median size: 111.7 nm and the proportion of the main peak was 94.1%. Visualised via TEM . , WB : CD63, TSG101, and HSP70 positive. Calnexin negative. , Microarray analysis of miRNA from sEVs and endometrial tissues from uterine aspirate fluid, followed by qRT-PCR . sEV uptake experiments with JNK activator anisomycin by M0 macrophages followed by flow cytometry , WB, and MAPK phosphorylation antibody array . Transwell migration and invasion assay : Co-culture of endometrial tissues of control group and sEV-treated macrophages. Transfection of M0 macrophages with hsa-miR-210-3p mimic/inhibitor/corresponding NC followed by qRT-PCR and flow cytometry . , ↑ hsa-miR-210-3p Regardless of secretory or proliferative phases , Jiang et al. (2022) .

Techniques: Expressing, Isolation, Concentration Assay, Western Blot, Microarray, Quantitative RT-PCR, Mouse Assay, Immunohistochemistry, Extraction, Transfection, Transmission Electron Microscopy, Sequencing, Flow Cytometry, Reporter Gene Assay, Zeta Potential Analyzer, Cell Culture, In Situ, Hybridization, Wound Healing Assay, Marker, Ligation, Migration, Size-exclusion Chromatography, Ab Array, Invasion Assay

Journal: Human Reproduction (Oxford, England)

Article Title: The role of small extracellular vesicle-miRNAs in endometriosis

doi: 10.1093/humrep/dead216

Figure Lengend Snippet: Studies investigating the differential expression of sEV-miRNA.

Article Snippet: 3 , Serum Eutopic endometrium Endometriotic lesion Endometriosis mouse model , Human samples : Endometriosis (ovarian endometrioma, from ovarian cystectomy or oopherectomy and confirmed with histology) and control (hysterectomy for other pelvic masses): *Serum, n=20 each Eutopic endometrium, n=unclear Endometriotic lesion, n=unclear *sEV source **Proliferative phase samples Samples from mice : Endometriosis mouse model, n=24, divided into 4 treatment groups , ExoQuick-TC Exosome Isolation Kit (System Biosciences SBI, USA) , TEM: 90–120 nm. NTA not done. , WB: CD9 and CD63 positive. , Immunohistochemistry and WB for CCN2, α-SMA, and collagen α1. miRNA extraction of all cell types and qRT-PCR for hsa-miR-214-3p. Transfection of all cell types with hsa-miR-214-3p/mimics/NC and qRT-PCR for miRNA and CCN2. Co-culture of transfected and untransfected cells followed by qRT-PCR for miRNA and CCN2. Serum sEV-miRNA: qRT-PCR . Endometriosis mouse model: sEV-miRNA uptake experiment , qRT-PCR . , ↓ hsa-miR-214-3p , Zhang et al. (2021) .

Techniques: Expressing, Isolation, Concentration Assay, Western Blot, Microarray, Quantitative RT-PCR, Mouse Assay, Immunohistochemistry, Extraction, Transfection, Transmission Electron Microscopy, Sequencing, Flow Cytometry, Reporter Gene Assay, Zeta Potential Analyzer, Cell Culture, In Situ, Hybridization, Wound Healing Assay, Marker, Ligation, Migration, Size-exclusion Chromatography, Ab Array, Invasion Assay

Journal: Human Reproduction (Oxford, England)

Article Title: The role of small extracellular vesicle-miRNAs in endometriosis

doi: 10.1093/humrep/dead216

Figure Lengend Snippet: Retrograde menstruation is only one part of the endometriosis pathophysiology. (1) Retrograde menstruation brings endometrial cells into the peritoneal cavity. (2) sEVs are produced by all cells (endometrial cells, red blood cells, pMφ, andPMCs) in the peritoneal cavity. (3) sEVs containing miRNAs are taken up by endometrial cells, red blood cells, pMφ, and PMC, causing changes to recipient cells. (4) Uptake of sEVs and internalisation of miRNAs promotes proliferation, migration, and invasion of endometrial cells and of existing ectopic lesions, immunomodulation of macrophages, and potentially EMT changes of PMCs, although none of the studies in this review investigated the impact of sEV-miRNA on PMCs. PF-derived sEVs are likely to originate from a variety of cell types including endometrial cells, red blood cells, immune cells, ectopic lesions, and PMCs. This figure was created with BioRender.com. sEV, small extracellular vesicle; miRNA, microRNA; pMφ, peritoneal macrophage; PMC, peritoneal mesothelial cell; EMT, epithelial-to-mesenchymal transition; PF, peritoneal fluid; sEV-miRNA, small extracellular vesicle-microRNA.

Article Snippet: 3 , Serum Eutopic endometrium Endometriotic lesion Endometriosis mouse model , Human samples : Endometriosis (ovarian endometrioma, from ovarian cystectomy or oopherectomy and confirmed with histology) and control (hysterectomy for other pelvic masses): *Serum, n=20 each Eutopic endometrium, n=unclear Endometriotic lesion, n=unclear *sEV source **Proliferative phase samples Samples from mice : Endometriosis mouse model, n=24, divided into 4 treatment groups , ExoQuick-TC Exosome Isolation Kit (System Biosciences SBI, USA) , TEM: 90–120 nm. NTA not done. , WB: CD9 and CD63 positive. , Immunohistochemistry and WB for CCN2, α-SMA, and collagen α1. miRNA extraction of all cell types and qRT-PCR for hsa-miR-214-3p. Transfection of all cell types with hsa-miR-214-3p/mimics/NC and qRT-PCR for miRNA and CCN2. Co-culture of transfected and untransfected cells followed by qRT-PCR for miRNA and CCN2. Serum sEV-miRNA: qRT-PCR . Endometriosis mouse model: sEV-miRNA uptake experiment , qRT-PCR . , ↓ hsa-miR-214-3p , Zhang et al. (2021) .

Techniques: Produced, Migration, Derivative Assay

Journal: Human Reproduction (Oxford, England)

Article Title: The role of small extracellular vesicle-miRNAs in endometriosis

doi: 10.1093/humrep/dead216

Figure Lengend Snippet: Proposed sEV-miRNAs involved in the pathophysiology of endometriosis. sEV-miRNAs involved in the pathophysiology of endometriosis through the proliferation, migration, and invasive potential of endometrial cells, immunomodulation, and angiogenesis (formation of blood vessels) to support explanted endometrial cells (ectopic lesions). Studies also investigated endometriosis-specific miRNAs and the signalling pathways involved. This figure was created with BioRender.com. sEV-miRNA, small extracellular vesicle-microRNA.

Article Snippet: 3 , Serum Eutopic endometrium Endometriotic lesion Endometriosis mouse model , Human samples : Endometriosis (ovarian endometrioma, from ovarian cystectomy or oopherectomy and confirmed with histology) and control (hysterectomy for other pelvic masses): *Serum, n=20 each Eutopic endometrium, n=unclear Endometriotic lesion, n=unclear *sEV source **Proliferative phase samples Samples from mice : Endometriosis mouse model, n=24, divided into 4 treatment groups , ExoQuick-TC Exosome Isolation Kit (System Biosciences SBI, USA) , TEM: 90–120 nm. NTA not done. , WB: CD9 and CD63 positive. , Immunohistochemistry and WB for CCN2, α-SMA, and collagen α1. miRNA extraction of all cell types and qRT-PCR for hsa-miR-214-3p. Transfection of all cell types with hsa-miR-214-3p/mimics/NC and qRT-PCR for miRNA and CCN2. Co-culture of transfected and untransfected cells followed by qRT-PCR for miRNA and CCN2. Serum sEV-miRNA: qRT-PCR . Endometriosis mouse model: sEV-miRNA uptake experiment , qRT-PCR . , ↓ hsa-miR-214-3p , Zhang et al. (2021) .

Techniques: Migration